plasmids 1864 1858 1856 Search Results


plasmids 1864 1858 1856  (Addgene inc)
93
Addgene inc plasmids 1864 1858 1856
Plasmids 1864 1858 1856, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/pmc09510660-120-16-15?v=Addgene+inc
Average 93 stars, based on 1 article reviews
plasmids 1864 1858 1856 - by Bioz Stars, 2026-07
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lentiviral vectors  (Addgene inc)
96
Addgene inc lentiviral vectors
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Lentiviral Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/10__1158_slash_0008___5472__can___13___0560-60-11-15?v=Addgene+inc
Average 96 stars, based on 1 article reviews
lentiviral vectors - by Bioz Stars, 2026-07
96/100 stars
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raptor 2  (Addgene inc)
93
Addgene inc raptor 2
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Raptor 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/pmc09510660-120-6-15?v=Addgene+inc
Average 93 stars, based on 1 article reviews
raptor 2 - by Bioz Stars, 2026-07
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mtor 2  (Addgene inc)
93
Addgene inc mtor 2
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Mtor 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/pmc09510660-120-7-15?v=Addgene+inc
Average 93 stars, based on 1 article reviews
mtor 2 - by Bioz Stars, 2026-07
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plasmids 12259  (Addgene inc)
98
Addgene inc plasmids 12259
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Plasmids 12259, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/pmc09510660-120-31-30?v=Addgene+inc
Average 98 stars, based on 1 article reviews
plasmids 12259 - by Bioz Stars, 2026-07
98/100 stars
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lentiviral plasmids pmd2 g  (Addgene inc)
98
Addgene inc lentiviral plasmids pmd2 g
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Lentiviral Plasmids Pmd2 G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/pmc08709853-211-24-32?v=Addgene+inc
Average 98 stars, based on 1 article reviews
lentiviral plasmids pmd2 g - by Bioz Stars, 2026-07
98/100 stars
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rictor 1 shrna  (Addgene inc)
93
Addgene inc rictor 1 shrna
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Rictor 1 Shrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/pmc08709853-211-9-32?v=Addgene+inc
Average 93 stars, based on 1 article reviews
rictor 1 shrna - by Bioz Stars, 2026-07
93/100 stars
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mtor 1 shrna  (Addgene inc)
93
Addgene inc mtor 1 shrna
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Mtor 1 Shrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/pmc08709853-211-15-32?v=Addgene+inc
Average 93 stars, based on 1 article reviews
mtor 1 shrna - by Bioz Stars, 2026-07
93/100 stars
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raptor 1 shrna  (Addgene inc)
93
Addgene inc raptor 1 shrna
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Raptor 1 Shrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/pmc08709853-211-3-32?v=Addgene+inc
Average 93 stars, based on 1 article reviews
raptor 1 shrna - by Bioz Stars, 2026-07
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gene pd  (23andMe)
90
23andMe gene pd
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Gene Pd, supplied by 23andMe, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/10__3233_slash_jpd___181471-421-69-68?v=23andMe
Average 90 stars, based on 1 article reviews
gene pd - by Bioz Stars, 2026-07
90/100 stars
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cryptococcus depauperatus  (Broad Institute Inc)
90
Broad Institute Inc cryptococcus depauperatus
Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc <t>lentiviral</t> reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.
Cryptococcus Depauperatus, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+1864+1858+1856/pmc08000046__jof___07___00207___s001-135-0-11?v=Broad+Institute+Inc
Average 90 stars, based on 1 article reviews
cryptococcus depauperatus - by Bioz Stars, 2026-07
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Image Search Results


Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc lentiviral reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.

Journal: Cancer Research

Article Title: Genetic and Pharmacologic Inhibition of mTORC1 Promotes EMT by a TGF-β–Independent Mechanism

doi: 10.1158/0008-5472.can-13-0560

Figure Lengend Snippet: Figure 1. Description and validation of the EMT readout designed for the screen. A, schematic representation of the pVFir and pERuc lentiviral reporter constructs used in this study and the expected flip between E-cadherin and vimentin expression in cells undergoing EMT or MET. B, E-cadherin and vimentin expression were assayed in MCF7 and MDA-MB-231 cells by immunoblotting. C, firefly and Renilla luciferase expression after transduction of the pVFir and pERuc vectors in the MCF7 (epithelial) and MDA-MB-231 (mesenchymal) cell lines. D, calculation of the EMTi for MCF7 and MDA-MB-231 with luciferase values obtained from C. E, validation of EMTi with the mammary epithelial cell line MCF10A undergoing EMT after treatment with increasing amounts of TGF-b for 48 hours. F, E-cadherin and vimentin expression in MCF10A cells treated for 72 hours with the indicated amount of TGF-b were evaluated by immunoblotting. Experiments were carried out in triplicate. Error bars, SD. LTR, long terminal repeat; LTRsin, LTR with deletion in the U3 region; VIM, vimentin; ECAD, E-cadherin; GAPDH, glyceraldehyde-3-phosphate deshydrogenase.

Article Snippet: Stable silencingofmTOR,RPTOR, and RICTOR was achieved after transduction of cells with lentiviral vectors obtained from Addgene (reference numbers 1864, 1855, 1856, 1857, 1858, 1853, and 1854; ref. 14).

Techniques: Biomarker Discovery, Construct, Expressing, Western Blot, Luciferase, Transduction